Journal: Translational Oncology
Article Title: β-Sitosterol enhances the anti-tumor efficacy of sorafenib in hepatocellular carcinoma via the FXR/LXR/ SREBP1/ FASN pathway
doi: 10.1016/j.tranon.2025.102610
Figure Lengend Snippet: Antitumor effects of SIT and combined treatment on HCC cells. HepG2 cells (A) and Hepa1–6 cells (B) were administrated SIT, sorafenib, and sorafenib + SIT at serial concentrations, and Cell Counting Kit-8 (CCK-8) assays were used to determine the cell viability. HepG2 cells (C) and Hepa1–6 cells (D) were treated with SIT (2 μM), sorafenib (10 μM) or a combination of both, and cell proliferation was evaluated using the 5-ethynyl-29-deoxyuridine (EdU) assay. HepG2 cells (E) and Hepa1–6 cells (F) were treated with SIT (2 μM), sorafenib (10 μM) or a combination of both, and cell apoptosis induction was evaluated using flow cytometry. Hepa1–6 cells (G) were treated with SIT (2 μM), sorafenib (10 μM) or a combination of both, and vasculogenic mimicry (VM) formation was evaluated in three-dimensional culture. Data were presented as mean ± SEM, n = 3, One-way ANOVA. * p < .05; ** p < .01; *** p < .001; and **** p < .0001.
Article Snippet: The human HCC cell line HepG2 and the murine HCC cell line Hepa1–6 was purchased from the American Type Culture Collection.
Techniques: Cell Counting, CCK-8 Assay, EdU Assay, Flow Cytometry